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Individual clones containing LacAnc100 and LaccID were inoculated into 50 l of SC minimal medium containing 6.7 g l 1 yeast nitrogen base (BD, 291940), 1.92 g l 1 yeast synthetic dropout medium supplement without uracil (Merck, Y1501), 20 g l 1 d-raffinose (Merck, R0250) and 25 g ml 1 chloramphenicol (Merck, C0378) in a sterile 96-well plate (Enzyscreen, CR1496c)

- Avoid exceeding 20 minutes, as tomato acids can irritate if overused

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In addition, IL-4 / mice had decreased production of tumor necrosis factor , CXCL1 and interleukin-1 in the liver, but not in a remote site such as the lungs

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