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A logical explanation for the post-infective syndrome is the initiation of autoreactive process affecting numerous functions including brain and metabolism

In addition, Daubry and colleagues demonstrated anxiogenic and inflammatory effects with 200 mg.kg -1 of caffeine in sleep-deprivation rats [37]

After 24 h of incubation, the supernatant was collected to quantify lactate dehydrogenase (LDH) release using the CyQuant LDH assay kit (Invitrogen, Cat #C20300), following the manufacturers protocol

Ethics approval and consent to participate All animal experiments were conducted in accordance with the guidelines approved by the Animal Experimentation Ethics Committee of Kyoto Pharmaceutical University (Approval No.: A21-011-02)