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Artificial metalloenzymes (ArMs) are defined as biocatalysts that incorporate an unnatural metal cofactor into a protein scaffold to elicit an advantageous effect (stereoselectivity, targetability, etc) over the free metal 45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65

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Tesamorelin's FDA approval reflects a completed phase 3 programme that no other GHRH analogue has undergone Clinical significance: the phase 3 trial data provides a more robust safety and efficacy evidence base than is available for most peptides, including defined adverse event rates, population characteristics, and CT-measured visceral fat outcomes Sourcing implication: Tesamorelin (Egrifta) is a licensed pharmaceutical product

NMR of the CsiD reaction The reaction of -ketoglutarate (10 mM) and glutarate (10 mM) catalysed by CsiD in 20 mM ammonium acetate buffer pH 7.25 was monitored by 1 H NMR spectra without purification

Dose and Frequency More frequent injections or higher doses require more vials, increasing costs

doi: 10.1038/onc.2017.37 70 ZhangYXiongXZhuQZhangJChenSWangYet al