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Cell adhesion and polysaccharide synthesis Bacterial biofilms are encapsulated with a self-produced extracellular polymer matrix (ECM), composed of polysaccharides (PS), eDNA, lipids, carbohydrates and proteins 192

On day 15, cells were confluent and differentiation was induced by adding 1.5% DMSO in the culture medium (differentiation medium)

Luque RM, Cordoba-Chacon J, Ibanez-Costa A, Gesmundo I, Grande C, Gracia-Navarro F, Tena-Sempere M, Ghigo E, Gahete MD, Granata R, Kineman RD, Castano JP

Supplementation also allows for a consistent dose each day

Actual Size RSC Adv ., 2021,11, 38161-38171 Can Med Assoc J

Then, 10 g of each purified extract was treated with DNAse to eliminate DNA contamination, and the RNA quality and yield were verified with Bioanalyzer chips using an Agilent RNA 6000 Nano Kit according to the manufacturers instructions