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To evaluate the physiological relevance of these new spines and to determine the neurotransmitter signature of resident synapses, HGF treatment-induced spines labeled with mRFP--actin were immunostained for the universal presynaptic marker Synapsin that is located in the presynaptic active zones (Ferreira and Rapoport 2002) and the excitatory presynaptic marker VGLUT1 that is found at glutamatergic presynaptic synapses (Balschun, Moechars et al.)

Sakaguchi S, Yamaguchi T, Nomura T, Ono M

doi: 10.1002/advs.202409711 85 LiM.LiuX.ChenW.XuH.HuangF.YaoQ.et al

[25] Liu Y, Yang Z, Kong D, Zhang Y, Yu W, Zha W

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