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In endothelial cells (ECs), copper overload disrupts mitochondrial dynamics, increases fission and ROS production, and ultimately leads to cell death [34] In cardiomyocytes, copper promotes hypertrophy and inflammation [35], disrupts fatty acid and lipid metabolism, upregulates autophagy-associated proteins and genes, and impedes calcium ion uptake by the sarcoplasmic reticulum, thereby inhibiting myocardial contractility [36]

In a rat model of HFD-induced MASLD, serum levels of IL-22 were markedly reduced, while the hepatic expression of the autophagy marker LC3B was increased

Randomized Cross-over Trial of Short-Term Water-Only Fasting: Metabolic and Cardiovascular Consequences

Sanchez-Barroso L, Apellaniz-Ruiz M, Gutierrez-Gutierrez G, Santos M, Roldan-Romero JM, Curras M, et al

Bulun SE, Yilmaz BD, Sison C, et al

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