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Moreover, mRNA expression and activity of GCL, also displayed circadian rhythms, which were lost with loss of CYC or PER, directly linking GSH synthesis and the circadian clock (19)

The supernatant of lysed cells was collected by centrifugation (13,000 g at 4 C for 30 min) and transferred to cleaned Ni-NTA agrose resin in 2 mL Eppendorf tubes

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References: Badrinath M et al (2014): Isoniazid Toxicity