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However, the impact of the washes on metabolite leakage in suspended cells or spheroids that require a centrifugation step must be thoroughly examined

Kalatzis, et al further looked at pH-mediated transport mechanisms and found that cystine transport is strongly affected by disruption of the transmembrane pH gradient and that cystinosin operates as an H + symporter

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Simmons RK, Stringfellow SA, Glover ME, Wagle AA, Clinton SM