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The upshot of all this is that citrulline may not be an "essential amino acid," but it acts like one

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EC4 cells were grown in high glucose DMEM supplemented with 10% fetal bovine serum and 1 each of glutamine, nonessential amino acids, and sodium pyruvate

The method of claim 91, wherein the antigen binding site comprises an antibody

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Fresh DMEM medium was then added, and the cells were exposed to various reagents