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After APAP overdose, cytokines, such as tumor necrosis factor-alpha (TNF-), interleukin-1 (IL-1), and interleukin-6 (IL-6), and chemokines, such as monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein 2 (MIP-2), were detected in the plasma of animals and patients

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Doherty JR, et al

Among its isoforms, NOX2 is predominantly expressed in Kupffer cells and is activated via toll-like receptor 4 (TLR4) signaling, resulting in superoxide production and pro-inflammatory cytokine release [113, 114]

These models also provide longer exposure times, which are usually not possible in 2D cell culture systems (92, 145)