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Apigenin ameliorates chronic mild stress-induced depressive behavior by inhibiting interleukin-1 production and NLRP3 inflammasome activation in the rat brain, Behav

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Vials stored at refrigeration temperature (28C) rather than frozen have shorter shelf lives approximately 36 months because residual moisture content below 5% still permits slow degradation

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After incubation, cells were washed and lysed, and radioactivity of the cell lysate was measured using liquid scintillation counting