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248 In addition, IgG can promote the senescence phenotype of macrophages and microglia, release inflammatory factors such as IL-6 and TNF-, form a systemic chronic inflammatory environment, and accelerate multiorgan aging

These processes are tightly regulated by the HPG axis, culminating in the production of spermatozoa from spermatogonial stem cells (SSC) (57)

This helps maintain receptor sensitivity and ensures long-term effectiveness

Methods: Male Wistar rats were subjected to a chow (CHOW) diet or a free-choice (fc) diet of either chow, saturated fat and liquid sugar (fcHFHS), chow and saturated fat (fcHF), or chow and liquid sugar (fcHS) for 4 weeks

For lipid reporter experiments, HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency), incubated for 48 h and imaged by confocal microscopy for membrane localized GFP

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