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For anterograde tracing of the PVN DMV circuit, rAAV-hSyn-Cre-mCherry-WPRE-hGH polyA (AAV2/1-hSyn-Cre-mCherry, AAV2/1, 1.03 10 13 vg/mL) virus was injected into the PVN and Cre-dependent rAAV-EF1-DIO-mCherry-WPRE-hGH pA (AAV-DIO-mCherry, AAV2/9, 5.31 10 12 vg/mL) virus was injected into the DMV of C57 mice

Our following data suggest that this cell death most likely is the recently described disulfidptosis 14 (see also Discussion section)

367,520 Duloxetine relieves pain by increasing the synaptic availability of serotonin and norepinephrine in descending pathways, enhancing endogenous pain modulation