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J Sports Sci 19, 831-7 (2001)

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Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3) cultured in LB medium, after induction with 0.2 mM IPTG overnight at 16 C, the bacteria were pelleted by centrifugation at 4000 g for 10 min

10.1016/j.phytochem.2017.08.004 12 ChenM

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Co-stimulation with IL-1 and TNF- induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system