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Figure 5 Comparison of the actual values of outputs, achieved from the interaction of the different levels of the four inputs, with predicted values achieved from the ANN models, showed the greatest measured and predicted values of the percentage of putative and PCR-verified transgenic tobacco plants were related to the interaction of the LB4404 strain of Agrobacterium cell density of 0.8 ( OD 600 = 0.8) 300 M of acetosyringone 20 min inoculation duration (Supplementary Tables 1, 2)

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