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showed that low serum vitamin B 12 levels were observed in individuals taking metformin at doses over 2000 mg/day, and a prevalence of mild neuropathy reaching 22.3% in the metformin-users group compared to 15.1% in the non-metformin users group, consistent with the results of previous studies in the literature [49]

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Using a combination of PCR with primers to the ends of the vector and Sal I/Not I restriction enzyme digestion of mini-prep DNA, it was determined that the average insert size of the library was greater the 3.5 Kb

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Thereafter, the BPs were treated with 8 mmol/L GSH (Yeasen, G5931120, China) in PBS (pH 7.4) at 37 C for 24 h