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This whole scenario triggers inflammasome activation, IL-1 and IL-18 releasing to recruit immune cells, and leads to a translocation of cardiolipin from the IMM to the outer mitochondrial membrane (OMM) in the mitochondria-associated membranes (MAMs) [120, 207209]

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Protein concentration was determined by BCA assay (Pierce) and, in the case of cell lysates, adjusted to a concentration of 0.3 mg/mL in blocking buffer (Ray Biotech

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Ferroptosis, initiated by iron-dependent lipid peroxidation, has the potential to promote inflammation via the release of DAMPs, which in turn can affect immune responses (Qi and Peng, 2023)

Ln a non-limiting exemplification, as illustrated in Figure 2, a first polymerase- based amplification reaction using a first set of primers, F 2 and Ri, is used to generate a blunt-ended product (labeled Reaction 1, Product 1), which is essentially identical to Product A