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To test this, we added ascites to CAOV3 cells for 16 hours, after which the ascites was removed, washed with PBS, and the cells were immediately treated with erastin and monitored for cell death for 72 hours (Fig

This approach provides: Stimulation for repair, regeneration, or metabolic action Recovery time to prevent receptor fatigue A rhythm that mirrors natural growth and rest cycles Reduced risk of overstimulation or tolerance Think of it like exercise: you wouldnt train the same muscle group every day without rest

In contrast to other classes, the isoenzymes of the , , and classes have an additional 9 helix