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Central and peripheral mechanism of MOTS-c attenuates pain hypersensitivity in a mice model of inflammatory pain

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One aspect of the invention pertains to the use of small molecule quinoline derived cations of Formula I for muscular therapy, wherein: R 1 is C 1-4 alkyl R 2 , R 3 , R 4 , and R 5 are independently selected from the group consisting of: H, C 1-4 alkyl, halogen-substituted C 1-4 alkyl, NR 9 R 10 , and CN R 6 is H or halogen R 7 is H, methyl, or NR 11 R 12 R 8 is H, C 1-4 alkyl, halogen-substituted C 1-4 alkyl R 9 , R 10 , R 11 , and R 12 are independently selected from H and C 1-4 alkyl

the reaction was initiated by adding 4 L of 25 M stock purified NNMT protein (final concentration of NNMT in the reaction was 200 nM) and incubated on a heat block at 37 C

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