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Impact of heat stress on health and performance of dairy animals: A review

Protein Extraction and Western Blot Analysis Influenza virus-infected cells were lysed with RIPA lysis buffer [20 mM TrisHCl pH 8, 150 mM NaCl, 1% Triton X-100, 0.1% sodium dodecyl sulfate (SDS), 0.5%] supplemented with phenylmethylsulfonyl fluoride, protease inhibitor mixture, and phosphatase inhibitor (Sigma- Aldrich, Milan, Italy) and total extract was analyzed by SDS-PAGE followed by western blotting with anti-G6PD (#12263 Cell Signaling), anti-NRF2 (#12721 Cell Signaling) anti-SIRT2 (MAB4358 R&D systems) and anti-influenza (AB1074 Merck Millipore, Darmstadt, Germany) antibodies

10.1105/tpc.110.079145 Plant Cell 153 SunY.LiH.HuangJ.-R

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