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20 l reactions contained 1 g DNA template, 1x reaction buffer, 2.5 mM of each rNTP (Roche), 40 U RNase inhibitor (Thermo Fisher Scientific), and 6 U Large Fragment of DNA Polymerase I (Thermo Fisher Scientific)

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Their effect on body composition in research is driven substantially through reduced energy intake and improved insulin dynamics, not through direct action on fat cells