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Cell-free Incubation of GSH with Itaconate and its Derivatives GSH (10 M) was incubated without (0 M) or with 1 mM or 10 mM itaconate, DI or OI for up to 4 h in 1 mL IB in wells of a 24-well dish at room temperature

In NSCLC tissues, there is an elevation in both the mRNA and protein levels of apolipoprotein C1 (APOC1)

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For Western blot analysis, 30 g protein per sample were mixed with loading buffer, denatured at 95 C for 5 min (PDHA1 and pPDHA1) or at 37 C for 20 min (OXPHOS complexes) and separated on a TGX Stain-Free Precast gradient gel (Bio-Rad Laboratories GmbH, Germany)

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