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smegmatis cells stained with PI dye (red) showed some dead cells after treatment with the small-molecule test drug, but no dead cells were observed during normal culture in 7H9-ADC media

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The stain was prepared by dissolving 0.5 g of Oil-Red-O in 100 mL of isopropanol, rocking for 3 days, diluting to 60% with milliQ-H 2 O, and filtering through a 0.45 m filter

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Its not that high for 30 days, but the rise effect can last for 30 days