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High levels of oxidative stress can lead to inflammatory conditions (diabetes, cardiovascular disease and autoimmune disease) and illnesses such as cancer

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cDNA synthesis and gene expression analysis by qRT-PCR Total RNA was extracted from leaf samples using the Invitrogen RNA Reagent kit according to the suppliers instructions

Ample evidence exists that all these conditionscardiovascular risk factors, metabolic syndrome, insulin resistance, increased oxidative stress and systemic inflammation are great promoters of CVD and CVD-related mortality