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Description

Reverse transcription was performed using 1 g of total RNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Part No

10.1080/003655200750023651 47 KamalH.Sadr-AzodiO.EngstrandL.BrusselaersN

Hows that work

Since HepG2 cells retain most of the biochemical and morphological properties of human hepatocytes and have been applied extensively in studying hepatic lipid metabolism in association with NAFLD [42,43,44], a cellular model of NAFLD was successfully developed using PA load in HepG2 cells in this study to further investigate the interaction between riboflavin deficiency and PA load [21, 45]