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Elucidating the mechanisms underlying melatonin biosynthesis and its regulation in reproduction will provide valuable insights into fertility processes and enable the development of innovative treatments for reproductive disorders and infertility (14)

Once anesthesia was induced using 5% isoflurane, cardiac blood collection was performed, followed by the rapid excision of prostate tissue, which was subsequently washed with pre-chilled 1.15% KCl solution

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Cuprizone stock solution was prepared fresh for each treatment

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Regardless of the host cell lines and the MSX concentration up to 50 M used for selection, the q mAb of most clones decreased during 30 passages