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Though there are a number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins for one-step purification with glutathione, can also protect against proteolysis

Meanwhile, in non-selenium containing Gpxs, which represent the majority of non-vertebrate Gpxs, the selenocysteine residue at the active site is replaced by a cysteine residue and the enzyme follows Michaelis Menten saturation kinetics (Toppo et al., 2009)

10.1523/JNEUROSCI.5491-10.2011 41 KawabataH.TongX.KawanamiT.WanoY.HiroseY.SugaiS.et al (2004)

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[DOI] [PubMed] [Google Scholar] 30.Fan PW, Bolton JL