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Head-to-Head: Which Is Better for Specific Goals

HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)

doi: 10.4274/EJBH.GALENOS.2021.2021-4-3 148 Canadian Cancer Society

While these compounds show promise in preclinical studies, their safety and efficacy in humans remain to be established through proper clinical trials

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