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However, it is limited by its qualitative or semi-quantitative nature, relatively low specificity, the need for precise primer design, and challenges arising from uneven 5mC distribution (62)

Behavioral tests, histological staining, oxidative stress markers, and protein expression were examined

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Samples were separated by SDS-PAGE (NuPage 412% Bis-Tris Gel, Life Technologies), run with BioRad Precision Plus Protein Kaleidoscope ladder, and transferred to a nitrocellulose membrane using an iBlot system (Life Technologies)