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Example Protocol Using the HisLink Resin to Purify Proteins from Cleared Lysate by Gravity-Flow Column Chromatography Materials Required: HisLink Protein Purification Resin (Cat.# V8821) and protocol HEPES buffer (pH 7.5) imidazole HisLink Binding Buffer HisLink Wash Buffer HisLink Elution Buffer column Cell Lysis: Cells may be lysed using any number of methods including sonication, French press, bead milling, treatment with lytic enzymes (e.g., lysozyme) or use of a commercially available cell lysis reagent such as the FastBreak Cell Lysis Reagent (Cat.# V8571)

Upon stimulation, eNOS produces NO, which diffuses to adjacent smooth muscle cells, leading to the activation of soluble guanylate cyclase (sGC)

Gilbert ES

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Cipolla D et al, Inhalable and nasal biologics: analytical, formulation, development, and regulatory considerations

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