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Routine bloodwork through a primary care provider can identify a deficiency early

Children presenting neurological signs or symptoms, those unable to take oral medication, individuals with known malabsorption syndromes, infants under 6 months old, and patients using medications that may interfere with vitamin B12 absorption are typically treated with a parenteral protocol (1)

While most adults get the recommended daily intake through diet alone, many Americans suffer from a chronic Vitamin B12 Deficiency

Following that, the sections were incubated in blocking medium containing 0.1% Triton X-100, 3% normal goat serum, and 2% bovine serum albumin for 1 h to block nonspecific binding

Thats a lot of extra puncture marks

34 , 19471959.e5 (2022)