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Molecular cloning of MAST4 and site-directed mutagenesis We used Phusion High-Fidelity DNA Polymerase to amplify the N-terminal (MAST4-N) and C-terminal (MAST4-C) halves of human MAST4 cDNA using the cDNAs of HEK293 cells as a template (primers are listed in Table S2)

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The combination of these two measures can reveal a link between cochlear neuropathy and central neural hyperactivity, providing characteristic evidence that cochlear synaptopathyseen as a reduction in wave-I amplitudeis followed by compensatory central gain, reflected in the relatively stable amplitude of wave V 11,45

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A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo