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Cells were washed twice in ice-cold PBS and lysed for 15min on ice with RIPA buffer (50mM Tris-HCl pH 8.0, 150mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with benzonase (25U/mL) and 1x Halt protease inhibitor cocktail

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