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At 24 h post-transfection, cells were lysed and subjected to pulldown using S-protein agarose beads, and the effect of PTPN22 on the interaction of AKT and mSIN was assessed by immunoblotting pulldowns with AKT specific antibody

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Toth P, Tarantini S, Ashpole NM, Tucsek Z, Milne GL, Valcarcel-Ares NM, et al