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For extracellular recordings of spontaneous action potentials, pipettes (57 M) were filled with (in mM), 130 NaCl, 2.5 KCl, 10 HEPES, 10 Glucose, 2 CaCl 2 , 1 MgCl 2 , pH 7.4 with NaOH (295 mOsm adjusted with NaCl)