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For instance, affinity chromatography is reputed for its high affinity, but tightly bound proteins typically require relatively extreme eluting conditions to disassociate from affinity columns

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A similar reduction occurred in the presence of anti-CD44 KM114 monoclonal antibody, which masks the hyaluronic acid-binding site, whereas an irrelevant human TRAIL (hTRAIL)Fc fusion had no effect (Fig

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