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2.5 Mass Spectrometry Detection Detection of metabolites was performed using a Thermo Q Exactive Focusmass spectrometer (ThermoFisher Scientific, USA) with both positive and negative ESI models, respectively

Signs that warrant medical attention: Erythema spreading beyond 5cm from the injection site, particularly if accompanied by warmth and swelling this pattern is consistent with early cellulitis Purulent discharge from the injection site Fever within 2448 hours of injection A firm, tender nodule that persists beyond 72 hours and is growing rather than resolving If you aspirate blood into the syringe before injection visible blood drawing back into the barrel when you pull the plunger slightly withdraw the needle, discard the syringe, and prepare a new one

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