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Clinical characteristics of airway impairment assessed by impulse oscillometry in patients with chronic obstructive pulmonary disease: findings from the ECOPD study in China

10.1016/j.drugalcdep.2014.07.013 32 HanK.-H.HashimotoN.FukushimaM

Kazemi et al., 2022)

Challenges and Solutions The use of GST-tagged proteins, while highly effective in many applications, comes with a set of challenges that researchers must address to optimize outcomes

In rats, GCLC has a Km for glutamate that is about 10-fold higher than that of the GCL holoenzyme, which is higher than the cellular glutamate concentration in most tissues GCL is specific for the glutamyl moiety and is regulated physiologically by: (a) non-allosteric feedback competitive inhibition by glutathione (GSH) (Ki = 2.3 mM) which involves binding of GSH to the glutamate and another site on the enzyme [8, 9] and (b) availability of its precursor, cysteine [1]