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Acute toxicity studies in rats have identified an oral acute toxicity (LD50) value of 2.506 mol/kg, suggesting that a significantly high dose is required to cause acute harm

10.1016/j.cbpa.2021.02.009 53

However, the longer and more variable telogen phase in females may introduce greater individual differences, potentially increasing data variability

The coverslips containing the cells were then washed with ice-cold PBS three times, fixed with 4% paraformaldehyde at 4 C for 30 min, permeabilized with Triton X-100 for 10 min, and subjected to three 5-min washes