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Using PASS 15.0 statistical software (NCSS LLC, Kaysville, UT, USA) for analysis, with a Type I error of 0.025 and a power (1-) of 0.8 (one-sided), and setting = 0, the required sample size was determined to be 23 participants

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The study found that serum B12 levels were comparable, and in some cases higher, among those using the sublingual supplement (1)

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Semaglutide directly stimulates GLP-1 receptors in the brain, decreasing appetite and changing satiety signals, and has a prolonged half-life of approximately seven days, enabling once-weekly administration