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50 mM NH 4 HCO 3 was added to centrifuge tube and inserted upside down into a new tube, centrifuged at 2500 g for 5 min, transferred protein solution to chromatographic sample bottles and added 2 mg/mL Trypsin into sample bottles, reacted at 37 C for 20 h

UFC903008), followed by centrifugation at 21,130 g for 15Lmin

Garca-Gimnez JL, Rom-Mateo C, Prez-Machado G, Peir-Chova L, Pallard FV (2017) Role of glutathione in the regulation of epigenetic mechanisms in disease

FIGURE 4 APC (adenomatous polyposis coli) encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway

However, due to the limited expression of ALT1/2 (or GPT1/2) and low transaminase activity, which restricts alanine biosynthesis, T cells primarily rely on extracellular alanine for protein synthesis (Ron-Harel et al., 2019)