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Description

Tissue clarity and morphological reconstruction The sections of the small intestine were cleared using three-dimensional imaging of solvent-cleared organs with superior fluorescence-preserving capability (FDISCO) protocol

The TEV-biotin tag contains three different components: a biotin group for streptavidin affinity purification, a TEV (Tobacco etch virus) protease recognition domain (ENLYFQG) conjugated to an isotopically labeled Valine (heavy or light), and an azide handle for click chemistry, and a linker between a reporter and the reactive group (Weerapana et al., 2007