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P values were determined by 2 tailed independent-samples t test, 1-way ANOVA with Bonferroni, Dunnett T3, Tukey HSD, and LSD posthoc tests, and 2-way repeated measures

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Mammalian Cells Cell culture Human embryonic kidney (HEK) cells were cultured in DMEM medium, containing 4.5 g/L glucose, 10 % FCS and 1 % penicillin/streptomycin, and cultivated at 37 C in a humidified atmosphere containing 5% CO 2

Medicina 58 (2), 319

1 2 "Histone H3 dopaminylation in nucleus accumbens, but not medial prefrontal cortex, contributes to cocaine-seeking following prolonged abstinence"

MMP, a key indicator of mitochondrial functionality, was assessed using JC-1 dye, which differentiates between energized and depolarized mitochondria