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B12 deficiency is significantly more common in India than in most Western populations, affecting an estimated 47-70% of vegetarians and a substantial proportion of the general population due to dietary patterns

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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work