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In this study, by detecting the ROS concentration in trophoblast cells co-cultured with the ferroptosis inducer erastin, ferroptosis inhibitor ferrostatin-1 (Fer-1), apoptosis inhibitor Z-VAD-FMK and necrosis inhibitor necrostatin-1 (Nec-1) and the concentrations of ferroptosis markers in the placental tissue of pregnant women with PE and pregnant rats, we confirmed that trophoblast cells are specifically sensitive to ferroptosis and that ferroptosis occurs in the pathological state of PE

All samples were dried under nitrogen, resuspended in 5% SSA, and analyzed via LC-HRMS as described

158 NETosis is a specific form of programmed cell death in which NADPH oxidase is activated when the concentration of Ca 2+ in the cytoplasm increases, leading to the massive production of ROS and ultimately inducing NETosis

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