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Osteogenic markers, including BMP-2, Osterix, Collagen type I and Osteocalcin, are upregulated to induce osteogenesis (82,83)

Consistent with an induction of oxidative stress condition, metformin increased mRNA levels of the master redox transcription factor Nrf-2 (nuclear factor erythroid-derived 2-like), as well as transsulfuration enzymes cystathionine beta-synthase and cystathionase and GSH synthesis enzymes -glutamylcysteine synthetase and glutathione synthetase

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For instance, in one 1960s study of intravenous NAD+ for addiction, patients who received a fast IV drip experienced immediate headache and shortness of breath, whereas those given the same dose at a slower drip rate (under ~35 drops/min) had no distress

[DOI] [PMC free article] [PubMed] [Google Scholar] 45.Satarug S., Gobe G.C., Vesey D.A