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Journal of Pre-Clinical and Clinical Research

A notable feature of the IGP synthase reaction is that it is a series of biochemical stepscondensation, decarboxylation and dehydration in that sequence, whose kinetic mechanisms have recently been elucidated (Schlee et al., 2013)

The mobile phase contained ammonium dihydrogen phosphate (75 mM), and the pH was adjusted to 5.7 using ammonia solution, which was then degassed in an ultrasonic bath for 10 min at room temperature