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Plasma was rapidly separated from cellular components by centrifuging at 3300 g at 4C for 15 min

For hPrdx6 protein purification, the crude protein extract was passed through the Ni-NTA agarose column pre-equilibrated with binding buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 10 mM Imidazole), followed by washing and elution using wash buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 60 mM Imidazole) and elution buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 500 mM Imidazole), respectively

Subsequently, the cells were treated with the respective IC 50 concentrations of compound 2 and DOX (used as the positive control in all assays)

Stover PJ, Field MS

Arachidonic acid: physiological roles and potential health benefitsa review

Heart disease : Alcohol can increase your risk of heart disease, including heart attack and stroke