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In a second round, we utilized the junctions observed in the first round, using the following parameters: --outSAMstrandField intronMotif --outSAMtype BAM SortedByCoordinate --outSAMmapqUnique 255 --outFilterMultimapNmax 1 --outReadsUnmapped Fastx --chimJunctionOverhangMin 20 --alignSoftClipAtReferenceEnds No --sjdbFileChrStartEnd *SJ.out.tab --limitSjdbInsertNsj=5000000 --sjdbInsertSave All

28 CardosoB

For example, when introduced via microparticle bombardment, cDNA encoding the arGST from Zea mays ( ZmBZ2 ) can complement mutations of the corresponding genes encoding arGST ( PhAN9 ) in Petunia spp., and vice versa