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Eight week old female and male mice, after anaesthetization with intraperitoneal injection of ketamine hydrochloride (100 mg/kg body weight, Thea), xylazine (10 mg/kg body weight, VMD), and the local anesthetic (Unicane 0.4%

Its involved in making adenosine triphosphate (ATP), which is involved in energy transfer within cells ( Its other crucial role is to serve as an antioxidant and protect cells from oxidative damage ( Excessive amounts of free radicals lead to oxidative damage, which can interfere with regular cell functioning

Endogenous nitric oxide (NO), a proinflammatory mediator, was shown to be a critical factor in seizure initiation and to promote seizures in mice (165)

Durham et al., 2000

The process takes just a few minutes, and there is no downtime, allowing you to continue your day immediately afterward

Sens Actuators B Chem 199:463469